Urine Culture & Sensitivity

Urine Culture & Sensitivity

Test: In vitro test for the qualitative determination of urine culture and sensitivity.
Submission Criteria: Specimen: Freshly voided urine

Minimum Volume: 3 mL


  1. Sterile plastic urine container, no preservative
  2. Gray-top urine tube, with preservative (boric acid)

**Specimen source must be included on the specimen label (Clean Catch, Random, First Morning, Catheter, Foley, Surgical (Sterile) or Other).

Storage and Stability:
Specimen Collection Media Stability at 20-25°C Room Temp. Stability at 2-8°C Refrigerated Temp. Stability at <-20°C Frozen
Fresh Catch Urine Sterile Container 2 Hours 24 Hours Unacceptable
Preserved Urine Boric Acid 48 Hours 48 Hours Unacceptable

*If the urine cannot be delivered to the laboratory within 2 hours of collection, refrigerate for up to 24 hours both during the hold period and during the transport. Boric Acid preservative should be used if specimen transport is greater than 24 hours. Fresh urine should be transferred to a boric acid tube within 2 hours and is stable at room temperature for up to 48 hours.

Rejection Criteria: Rejection criteria include but are not limited to:

  1. Mismatched requisitions.
  2. Specimens without two patient identifiers.
  3. Specimens stored or shipped incorrectly.
  4. Specimens collected using expired preservative tubes.
  5. Specimens with inappropriate preservatives such as formalin or formaldehyde, disinfectant, or detergent added.
  6. Specimens not analyzed within the appropriate time frame.
  7. Specimens with quantity not sufficient.
  8. Hematuria
  9. Specimens contaminated with fecal matter.
  10. Specimens submitted without approval.
  11. Samples not shipped at appropriate temperature.
  12. Samples without two proper identifiers or samples having identifiers that do not match the electronic or paper lab requisition.
Turnaround Time:  2 business days. Test may be delayed if repeat testing or send out testing is required.
Clinical Significance: Urinary tract infections (UTI) are extremely common and encompass a broad range of clinical entities that vary in their clinical presentation, degree of tissue invasion, epidemiologic setting, and antibiotic therapy requirements. There are 4 major types of UTIs: urethritis, cystitis, acute urethral syndrome, and pyelonephritis. The classic symptoms of upper UTI are fever (often with chills) and flank pain. Frequent painful urination, urgency, and dysuria are more often associated with lower UTI.
Reference Ranges: No growth (Organism present <10,000 cfu/mL, or mixed microbiota.) Identification of probable pathogens with colony count ranges.
Method: Culture and Sensitivity

Bruker MALDI-TOF; BD Phoenix