Nail Biopsy for Pathology/Histology and PCR Analysis

Nail Biopsy for Pathology/Histology and PCR Analysis

Test Name: Higher Sensitivity panel for nail dystrophy/fungus
Routine PAS panel for nail dystrophy/fungus
Nail Neoplasia panels
Nail PCR
Method Name: Histology Panels are comprised of one or more of the following:
Hematoxylin and Eosin Stain
Periodic Acid – Schiff Stain
Grocott’s Methenamine Silver Stain
Gram’s Stain
Fontana Masson Stain
Immunohistochemical Staining

PCR Panels are comprised of the following targets:
Acremonium strictum, Alternaria spp., Aspergillus fumigatus, Aspergillus niger, Aspergillus terreus, Aspergillus versicolor, Candida albicans, Candida parapsilosis, Epidermophyton floccosum, Fusarium solani, Geotrichium candidum, Microsporon, audouinii, Microsporum, canis, Microsporum gypseum, Microsporum nanum, Natrassia magiferae, Pichia onychis, Sarcoptes scabiei, Scopulariopsis brevicaulis, Trichophyton interdigitale, Trichophyton rubrum, Trichophyton soudanense, Trichophyton tonsurans, Trichophyton violaceum, Trichosporon beigelii, Trichosporon mucoides

Results: Differential diagnoses include, but are not limited to: lichen planus, melanonychia, microtrauma, nail psoriasis, onychogryphosis, onycholysis, onychomycosis, melanoma, nail bed dystrophy, nail dystrophy
Reference Ranges: Not Detected for all targets
Clinical Significance: Diagnosis and prognosis of clinically ambiguous nail lesions or dystrophies.
Submission Criteria: Nail clip and excisional biopsies, nail removals: at least 6 mm of total tissue, dry or in formalin. Formalin submission is preferable when examination of nail bed or matrix is desired.
Nail punch biopsies: 2-3 mm punch in formalin.
Rejection Criteria: Rejection criteria include but are not limited to those with:

  1. Mismatched requisitions
  2. Specimens without TWO patient identifiers
  3. Specimens submitted without approval
Authorization: Diagnostic testing can only be performed with approval from an authorized provider/agency.
Turn Around Time: Histology: 5 Days
PCR Results: 72 Hours
Histology w/ PCR: 5 Days