Aerobic and Anaerobic Wound Culture

Aerobic and Anaerobic Wound Culture

Test: In vitro test for the quantitative determination of aerobic wound culture with gram stain.
Submission Criteria: Specimen source must be included on the specimen label as well as date and time of collection. Specimen must arrive within 48 hours of collection. Preferably collect specimen prior to institution of therapy.

Swab/Container: ESwab with ESwab transport medium (Amies Buffer)

Storage and Stability:
Specimen Stability at 20-25°C Stability at 2-8°C Stability at <-20°C
Collection Media Room Temp. Refrigerated Temp. Frozen
ESwab (Amies Buffer) 48 Hours 48 Hours Unacceptable
Rejection Criteria: Rejection criteria include but are not limited to:

  1. Specimens submitted with mismatched, incorrect, or blank requisition forms.
  2. Leaking specimens.
  3. Substandard mixing or collection.
  4. Expired or improperly stored collection tubes
  5. Improperly filled tubes based on collection tube manufacture’s guidelines.
  6. Specimens which arrive at the lab past the 48-hour mark
  7. Specimens submitted without a signed patient consent for treatment prior to specimen collection.
  8. Samples not shipped or stored at appropriate temperature.
  9. Samples without two proper identifiers or samples having identifiers that do not match the electronic or paper lab requisition.
Turnaround Time: 3-5 Business Days. Test may be delayed if repeat testing or send out testing is required.
Clinical Significance: Anaerobic bacteria area significant component of the normal microbiota of the human host. There are anaerobes on most body surfaces and mucous membranes; they exist in large numbers throughout the entire gastrointestinal tract from mouth to colon. Anaerobes can cause infection when they are introduced into a new site in the body (endogenous infections) or when nonnormal microbiota anaerobic bacterium gains entrance into the host.
Reference Ranges: No growth, Normal Skin Microbiota; Identification of probable pathogens.
Method: Culture and Sensitivity with Gram stain
Platform: Bruker MALDI-TOF; BD Phoenix